Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum.
نویسندگان
چکیده
Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody to the purified antigen. The availability of these reagents will enable the development of a sensitive and specific assay for detecting serologic reactivity to this antigen.
منابع مشابه
Immunoaffinity isolation and partial characterization of the Coccidioides immitis antigen detected by the tube precipitin and immunodiffusion-tube precipitin tests.
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ورودعنوان ژورنال:
- Infection and immunity
دوره 55 1 شماره
صفحات -
تاریخ انتشار 1987